Chromium in PDB 1huz: Crystal Structure of Dna Polymerase Complexed with Dna and Cr-Pcp

All present enzymatic activity of Crystal Structure of Dna Polymerase Complexed with Dna and Cr-Pcp:
2.7.7.7;

The structure of Crystal Structure of Dna Polymerase Complexed with Dna and Cr-Pcp, PDB code: 1huz was solved by J.W.Arndt, W.Gong, X.Zhong, A.K.Showalter, J.Liu, Z.Lin, C.Paxson, M.-D.Tsai, M.K.Chan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.60
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	105.780, 56.134, 85.414, 90.00, 106.94, 90.00
R / Rfree (%)	22.4 / 28.6

The binding sites of Chromium atom in the Crystal Structure of Dna Polymerase Complexed with Dna and Cr-Pcp (pdb code 1huz). This binding sites where shown within 5.0 Angstroms radius around Chromium atom.
In total 2 binding sites of Chromium where determined in the Crystal Structure of Dna Polymerase Complexed with Dna and Cr-Pcp, PDB code: 1huz:
Jump to Chromium binding site number: 1; 2;

Chromium binding site 1 out of 2 in the Crystal Structure of Dna Polymerase Complexed with Dna and Cr-Pcp


Mono view


Stereo pair view

A full contact list of Chromium with other atoms in the Cr binding site number 1 of Crystal Structure of Dna Polymerase Complexed with Dna and Cr-Pcp within 5.0Å range: probe atom residue distance (Å) B Occ A:Cr999 b:45.9 occ:1.00 O6 A:MDN338 1.9 54.4 1.0 OD1 A:ASP190 1.9 47.7 1.0 OD1 A:ASP192 2.0 43.6 1.0 O2 A:MDN338 2.0 55.2 1.0 O3 A:MDN338 2.9 58.0 1.0 CG A:ASP192 3.0 39.6 1.0 OP1 P:DT8 3.1 40.6 1.0 P1 A:MDN338 3.1 50.3 1.0 CG A:ASP190 3.1 45.6 1.0 P5 A:MDN338 3.2 55.0 1.0 OD2 A:ASP192 3.4 37.2 1.0 C4 A:MDN338 3.5 50.3 1.0 O A:ASP190 3.7 44.5 1.0 OD2 A:ASP190 3.7 37.3 1.0 O7 A:MDN338 4.0 58.4 1.0 C A:ASP190 4.1 46.5 1.0 N A:ASP190 4.2 48.8 1.0 CB A:ASP192 4.2 37.9 1.0 O5' P:DT8 4.2 42.4 1.0 P P:DT8 4.3 42.0 1.0 O8 A:MDN338 4.3 50.7 1.0 CB A:ASP190 4.3 45.2 1.0 CA A:ASP190 4.4 47.2 1.0 O1 A:MDN338 4.5 52.9 1.0 CA A:GLY179 4.5 42.8 1.0 C5' P:DT8 4.6 37.9 1.0 N A:ASP192 4.7 42.5 1.0 N A:MET191 4.8 46.5 1.0 N A:SER180 4.8 46.1 1.0 O3' P:DC7 5.0 40.5 1.0

Chromium binding site 2 out of 2 in the Crystal Structure of Dna Polymerase Complexed with Dna and Cr-Pcp


Mono view


Stereo pair view

A full contact list of Chromium with other atoms in the Cr binding site number 2 of Crystal Structure of Dna Polymerase Complexed with Dna and Cr-Pcp within 5.0Å range: probe atom residue distance (Å) B Occ B:Cr999 b:57.4 occ:1.00 O6 B:MDN338 1.8 63.3 1.0 O2 B:MDN338 1.9 63.2 1.0 OD1 B:ASP190 2.0 55.8 1.0 OD1 B:ASP192 2.1 53.6 1.0 OP1 D:DT8 2.2 43.2 1.0 P5 B:MDN338 3.1 65.7 1.0 CG B:ASP192 3.1 51.4 1.0 CG B:ASP190 3.2 53.2 1.0 P1 B:MDN338 3.2 64.2 1.0 O B:ASP190 3.4 56.9 1.0 C4 B:MDN338 3.5 62.9 1.0 OD2 B:ASP192 3.5 47.2 1.0 P D:DT8 3.5 39.8 1.0 OD2 B:ASP190 3.8 54.1 1.0 O5' D:DT8 3.9 39.4 1.0 C B:ASP190 4.0 55.4 1.0 O3 B:MDN338 4.0 66.8 1.0 O7 B:MDN338 4.1 66.1 1.0 O8 B:MDN338 4.1 63.0 1.0 N B:ASP190 4.1 54.3 1.0 O1 B:MDN338 4.3 66.0 1.0 CB B:ASP190 4.3 52.4 1.0 CA B:ASP190 4.4 54.7 1.0 OP2 D:DT8 4.4 41.9 1.0 CB B:ASP192 4.4 50.0 1.0 C5' D:DT8 4.7 35.7 1.0 O3' D:DC7 4.7 34.3 1.0 N B:ASP192 4.7 50.8 1.0 CA B:GLY179 4.8 55.0 1.0 OD2 B:ASP256 4.9 47.5 1.0 N B:MET191 4.9 55.5 1.0

J.W.Arndt, W.Gong, X.Zhong, A.K.Showalter, J.Liu, C.A.Dunlap, Z.Lin, C.Paxson, M.D.Tsai, M.K.Chan. Insight Into the Catalytic Mechanism of Dna Polymerase Beta: Structures of Intermediate Complexes. Biochemistry V. 40 5368 2001.
ISSN: ISSN 0006-2960
PubMed: 11330999
DOI: 10.1021/BI002176J